FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell16.35200000095197.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell16.35200000095197.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902081
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA6150.030.3616665
CTTCCGA32700.028.2071191
TCCGATC32700.028.138323
CCGATCT32650.027.9747034
TTCCGAT33500.027.3320522
CGATCTG15800.025.6293775
CGATCTT6400.021.0908415
CGATCTC10050.019.2510785
TACCGTC2350.018.1889827
GATCTAG3500.017.9975196
ATACCGT2650.017.827736
TCTTCCG13800.017.4432475
ATCACGC1305.7894795E-717.30712528
TAGCGTA1601.617991E-816.8726737
CGTCGTA2301.8189894E-1216.62814110
ATAGCGT1652.3648681E-816.3613826
ACCGTCG2351.8189894E-1216.2743518
GCTCTTC14950.016.251943
GTATATA8650.016.1249431
GTATTAG6100.015.8584691
Produced by FastQC (version 0.11.2)