FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell15.3520000009517d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell15.3520000009517d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544739
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA7200.028.4331555
TCCGATC41650.027.384773
TTCCGAT42950.026.6082732
CCGATCT42850.026.3553644
CTTCCGA44800.025.7600671
CGATCTG21000.025.4961035
CGATCTT7850.021.780115
GACGTGT10750.020.50849310
CTACGCT706.9938484E-419.2827684
ACGTGTG10800.019.16411411
GAGACGT11950.018.6373188
ACCGTAC851.2058565E-418.52803617
AGACGTG11900.017.5813479
AACCGTA1053.117334E-517.14124916
ACGCGTG800.001708642516.87938540
CGTGTGC13550.016.27089512
CGATCTC13750.016.0339135
GGAGACG15250.015.6369547
ATAGGGG4750.015.629193
GGGAGAC15500.015.2396076
Produced by FastQC (version 0.11.2)