FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell14.35200000095147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell14.35200000095147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018972
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA25610.12684673190118534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA6050.029.7468935
TCCGATC38600.029.198293
CCGATCT38800.028.9318244
TTCCGAT39500.028.5899642
CTTCCGA40200.028.260011
CGATCTG18100.028.2133245
CGATCTT8400.023.2995195
GTCGACG602.4601512E-422.51113943
CGATCTC10700.022.2858435
CGTCGAC754.592578E-521.00727342
TTAACGG2800.020.894435
GAGACGT9650.020.7476888
CGGTACC3100.020.33213845
GACGTGT8950.019.85688210
CGGTCGA600.00651497818.74859820
ACGTGTG9600.018.04373611
CCGTCGA750.001108225418.00578941
GGAGACG11700.017.8815047
AGACGTG10350.017.6056339
CCGTCGG2850.017.37357740
Produced by FastQC (version 0.11.2)