FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell13.3520000009513a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell13.3520000009513a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688336
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG15950.029.3376755
TCCGATC33250.028.4848213
TTCCGAT34250.027.7845172
CCGATCT34100.027.774794
CTTCCGA34450.027.6885191
CGATCTA6200.026.488315
ATCTGCG2100.020.3543577
CGATCTT5950.020.0392745
CGTCTAA600.00650574818.75298540
GACGTGT6600.018.74743310
GAGACGT7100.018.6946228
CGATCTC10800.018.1225195
ACGTGTG6850.018.06322111
TATATCG1253.8413782E-717.9975365
TATCGTT901.8682853E-417.4976047
TGCCTCG1305.7948455E-717.30532310
CGTCGTA1752.6975613E-916.71199810
GATCTGC8600.016.4803036
AGACGTG7650.016.1742559
GATCTAG3350.016.1171976
Produced by FastQC (version 0.11.2)