FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell12.35200000095110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell12.35200000095110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215685
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3850.17850105477896006No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG253.88189E-544.99675436
ATCTATA207.023025E-444.99675426
ACCCGTT253.88189E-544.99675421
AAGATGA207.023025E-444.99675419
TCCGATG501.0732037E-940.49707822
CCGATGT451.917033E-839.99711623
ATACGCG403.4365075E-739.38129438
GAATCTT301.1357796E-437.50599345
GGGAGGA301.1373317E-437.49729535
CGGATAC301.1373317E-437.49729535
CGGTATA301.1373317E-437.49729534
GACCTAC301.1373317E-437.49729525
GTTATAC552.728484E-936.81552535
CCGACCT1300.036.34353623
CTACTCT250.002099281936.01410344
CACGCGT250.002099281936.01410339
GTCTAAG250.002099281936.01410339
GCTGACT250.002099281936.01410342
CATTTGC250.002099281936.01410341
TGTCCAC250.002099281936.01410343
Produced by FastQC (version 0.11.2)