FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell11.352000000950f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell11.352000000950f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222890
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11450.5137063125308449No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT453.8198777E-1044.99843221
CTCGTAT207.0222444E-444.9984328
CTTTTGA207.0222444E-444.9984320
TATCTCG207.0222444E-444.9984325
TCAGCCG207.0222444E-444.9984329
CGGTATA302.1581218E-644.9984334
GTTATAC351.206372E-744.9984335
ACGGTGA253.881457E-544.9984334
ACGCGTC451.913213E-840.0075840
TACGCGT403.4363984E-739.3824639
TGTTATA356.227143E-638.57008434
GCTTACT301.1357031E-437.50710742
TCTGCAT301.13720496E-437.4986927
TCCGCCG601.546141E-1037.4986929
CTGCGTT301.13720496E-437.4986928
TCTGTGT301.13720496E-437.4986927
CCGGTAT301.13720496E-437.4986933
TCCGGTA301.13720496E-437.4986932
CTGATCT1550.036.28905523
GTCTACT751.8189894E-1236.00682442