FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell10.352000000950db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell10.352000000950db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222533
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAA207.010822E-445.01326443
CGGTATA253.8790313E-545.00314734
CTCCACT207.0185936E-445.00314328
TTAGGAC207.0263713E-444.9930343
GACCGTT207.0263713E-444.9930347
CTACGTT207.0263713E-444.9930349
ACCGTTA207.0263713E-444.9930348
TAGGACC207.0263713E-444.9930344
GGTTTGC356.213011E-638.58279441
CGACTCT700.038.58279444
CGGATAC301.13499336E-437.5110535
GCTTACT301.13499336E-437.5110542
GAACAAC301.13800226E-437.4941946
GTCTACT552.7193892E-936.82903742
GCCGCTT250.00210042436.01061239
CAGGTAT250.002102743436.00251833
TATGGGC250.002102743436.00251832
CTGACCT250.002102743436.00251828
TGAGGGC250.002102743436.00251832
TTCTCGC250.002102743436.00251826
Produced by FastQC (version 0.11.2)