FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell1.35200000094fb5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell1.35200000094fb5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923232
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA13850.15001646390073134No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGG10130.10972323316349519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC16400.028.5280343
CTTCCGA16450.028.3045861
TTCCGAT16750.027.7976362
CCGATCT16950.027.3369394
AAGACCG507.1715156E-526.9919075
CGATCTG7800.026.8188825
GTACTAG551.3707032E-424.53811
CGATCTA2350.023.9289995
TCTTCCG7400.022.797225
CGATCTC5450.021.8741765
TATATCG654.2459398E-420.7630085
CGATCTT3950.020.5001835
GCTCTTC8750.020.0511323
GTGCTCT8650.020.02291
TTTCGGG2150.019.885331
GGATACG706.9961394E-419.2799341
GGTTTCG2350.019.15045729
CTCCGAT600.0064943618.75758739
GATCGGA1951.8189894E-1218.4560051
ACCCGTC1608.1672624E-1018.2777515
Produced by FastQC (version 0.11.2)