FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell96.35100000095b83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell96.35100000095b83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22353
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGA330114.767592716861271TruSeq Adapter, Index 1 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC267711.976021115733907No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC19308.634187804768935TruSeq Adapter, Index 1 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTG18168.124189146870666RNA PCR Primer, Index 1 (96% over 25bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTT10334.621303628148347TruSeq Adapter, Index 1 (96% over 28bp)
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAA8313.7176217957321165RNA PCR Primer, Index 1 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT6002.6842034626224667RNA PCR Primer, Index 40 (95% over 22bp)
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGC3131.4002594730013869RNA PCR Primer, Index 40 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT2941.3152596966850088RNA PCR Primer, Index 1 (95% over 22bp)
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCT2301.0289446606719457RNA PCR Primer, Index 40 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGAACTCGTATGCCGTC1180.5278933476490851No Hit
ATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTG1170.523419675211381RNA PCR Primer, Index 40 (96% over 30bp)
TCTCCGAGCCCACGAGACATCTCAGGACCTCGTATGCCGTCTTCTGCTTGA800.3578937950163289Illumina Single End Adapter 1 (96% over 25bp)
TCTCCGAGCCCACGAGACATCTCAGGAACTCGTATGCCGTCTTCTGCTTGA800.3578937950163289Illumina Single End Adapter 1 (96% over 25bp)
CTTTACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT700.31315707063928777RNA PCR Primer, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGAACTCGTATGCCGTCTTCTG700.31315707063928777Illumina Single End Adapter 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGACCTCGTATGCCGTC670.2997360533261755No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGAACTCGTATGCCGTCTTC650.2907887084507672No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGACCTCGTATGCCGTCTTCTG610.2728940186999508Illumina Single End Adapter 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGAGCTCGTATGCCGTC570.25499932894913435No Hit
TCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAA530.2371046391983179RNA PCR Primer, Index 40 (96% over 30bp)
TCTCCGAGCCCACGAGACATCTCAGGAGCTCGTATGCCGTCTTCTGCTTGA510.22815729432290968Illumina Single End Adapter 1 (100% over 25bp)
GCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT440.19684158725898088No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGACCTCGTATGCCGTCTTC410.18342056994586856No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGAGCTCGTATGCCGTCTTC390.17447322507046034No Hit
TCTCCGAGCCCACGAGACATCTCAGGATATCGTATGCCGTCTTCTGCTTGA370.1655258801950521Illumina PCR Primer Index 1 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATATCGTATGCCGTC340.15210486288193978No Hit
CATCTCCGAGCCCACGAGACATCTCAGGAACTCGTATGCCGTCTTCTGCTT330.14763119044423567Illumina Single End Adapter 1 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGAGCTCGTATGCCGTCTTCTG300.13421017313112335Illumina Single End Adapter 1 (100% over 21bp)
CTTATAACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT250.11184181094260279No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACATCTCAGG250.11184181094260279No Hit
TATCACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC240.10736813850489868TruSeq Adapter, Index 1 (95% over 23bp)
CTTAATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT240.10736813850489868No Hit
CATCTCCGAGCCCACGAGACATCTCAGGACCTCGTATGCCGTCTTCTGCTT240.10736813850489868Illumina Single End Adapter 1 (95% over 24bp)
GATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT230.10289446606719457RNA PCR Primer, Index 40 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC3450.043.043481
TTATACA3800.039.0789452
GCTTGAA1150.037.17391245
TCAGGAG250.002063303536.029
TGCTTGA5050.034.75247645
CTGCTTG5150.034.5145644
TATACAC4400.033.7500043
TCTGCTT6050.029.38016343
TTCTGCT6450.027.5581442
CTTCTGC6750.026.33333441
ATACACA6700.022.1641774
TACACAT6850.021.6788315
GTCTTCT9000.020.039
TCTTCTG8950.019.86033440
ACACATC9150.016.475416
CGTCTTC11350.016.05726838
CACATCT9450.015.9523827
CCGTCTT11850.015.37974637
GCCGTCT12400.014.6975836
CATCTCC10750.014.0232569
Produced by FastQC (version 0.11.2)