FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell95.35100000095b69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell95.35100000095b69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794518
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGA122630.6833589855326054TruSeq Adapter, Index 8 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC121980.6797368429851358No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC96400.537191602424718TruSeq Adapter, Index 8 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTG60110.33496459773599374RNA PCR Primer, Index 8 (96% over 25bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTT31530.17570177618725474TruSeq Adapter, Index 8 (96% over 28bp)
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAA30890.17213535890974624RNA PCR Primer, Index 8 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT30760.17141093040025232TruSeq Adapter, Index 8 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGC19030.10604518873591684TruSeq Adapter, Index 8 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC19150.033.5093771
CTTCCGA38700.027.0540471
TCCGATC39100.026.5277063
TTCCGAT39550.026.339652
TGCTTGA24700.026.23439645
CCGATCT39900.025.9394344
CGATCTG18350.025.7489035
TTATACA25350.025.7392312
CGATCTA6700.025.1861635
GGTATAC450.00122176625.0177211
TATACAC26050.024.7020993
CTGCTTG26850.024.63647744
CCCAACG651.5245601E-524.23038121
GCTTGAA9600.022.03089545
CGGTACC1301.2296368E-920.76889845
CGATCTT7750.020.6125725
CGATCTC11650.020.2786435
TCTGCTT33350.019.96969443
GGCGTTA2250.018.99969542
TACACCG951.3166871E-518.9470635
Produced by FastQC (version 0.11.2)