FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell94.35100000095b5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell94.35100000095b5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377370
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC87350.634179632197594No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA84720.615085271205268TruSeq Adapter, Index 3 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC75750.5499611578588179TruSeq Adapter, Index 3 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG45050.327072609393264RNA PCR Primer, Index 3 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT26410.1917422333868169No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT23540.1709054212012749TruSeq Adapter, Index 3 (96% over 27bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA19930.14469605116998338No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA18170.13191807575306563RNA PCR Primer, Index 3 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC14210.10316763106500068TruSeq Adapter, Index 3 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA4300.032.965465
CTTCCGA31950.029.7358931
TTCCGAT32050.029.6258952
CGATCTG14350.029.3208665
TCCGATC32500.029.215693
CCGATCT32600.029.0570534
CTTATAC15900.028.3186321
TGCTTGA15300.025.4405245
CTGCTTG15600.025.09550744
TATACAC19450.024.0619493
CGATCTC10000.023.6252485
TTATACA19200.023.2033672
CGATCTT6650.023.0077615
GATCTCG4750.019.8949456
ATCTGCG2300.019.5647137
TCTGCTT20800.019.2543143
ACGGTAC600.006510744318.75019628
TCTTCCG12700.018.6025565
GGCGTTA2100.018.21381442
GCTCTTC13450.017.3979533
Produced by FastQC (version 0.11.2)