FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell93.35100000095b26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell93.35100000095b26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145664
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC2248115.433463312829526No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC1555710.680058216168717RNA PCR Primer, Index 33 (95% over 23bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA142229.76356546572935RNA PCR Primer, Index 33 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG97316.6804426625659055RNA PCR Primer, Index 33 (96% over 25bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT46083.163444639718805RNA PCR Primer, Index 33 (96% over 28bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAA35282.4220123022847098RNA PCR Primer, Index 33 (96% over 29bp)
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC28061.9263510544815465RNA PCR Primer, Index 15 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT28041.9249780316344465No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT19091.3105503075571177RNA PCR Primer, Index 33 (95% over 22bp)
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT12980.8910918277680141RNA PCR Primer, Index 15 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTC6900.4736928822495606No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG4810.3302119947275923RNA PCR Primer, Index 15 (96% over 28bp)
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT4640.3185413005272408RNA PCR Primer, Index 33 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTC4430.30412456063268895No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGTC4390.30137851493848855No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGCA4000.27460456942003514No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGTCTTC3080.21144551845342704No Hit
TCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCTTGA2970.2038938927943761Illumina PCR Primer Index 4 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGTC2960.20320738137082603No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTG2570.1764334358523726Illumina PCR Primer Index 4 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTC2470.1695683216168717No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGTCTTC2150.1475999560632689No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGTCTTCTG1790.12288554481546574Illumina PCR Primer Index 4 (95% over 22bp)
TCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGTCTTCTGCTTGA1780.12219903339191565Illumina PCR Primer Index 4 (96% over 26bp)
TATCACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC1660.1139608963093146RNA PCR Primer, Index 33 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAA253.8509934E-545.045361
TTATAAC253.874517E-544.998972
CTTGAAA207.013783E-444.9989745
CTTATAC26400.044.192231
GACACAT603.6379788E-1241.24905820
GCTTGAA5550.040.5396145
TTATACA29700.039.3930362
CAGACAT356.2117724E-638.5705520
TATACAC31200.037.499143
TTTACAC552.7157512E-936.817342
CTTATCA250.002090714936.036291
CTCGGAG250.002090714936.036291
CTGAGTT250.002101321535.9991764
CCACGAA250.002101321535.99917620
CCTGAGT250.002101321535.9991763
CAGATCT250.002101321535.99917622
TCTCCTA250.002101321535.99917615
AGACACA702.0008883E-1135.35633519
GACATCT401.5515076E-533.74922622
TGCTTGA21000.033.6420945
Produced by FastQC (version 0.11.2)