FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell92.35100000095b0c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell92.35100000095b0c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944435
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC38590.40860408604086035No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC33680.35661533085919095TruSeq Adapter, Index 10 (95% over 22bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA33330.3529094114470556TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG18390.19471959425476607RNA PCR Primer, Index 10 (95% over 24bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA15990.16930757542869546No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT10790.11424820130554246No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG9900.10482457765754129No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC9490.10048335777475421No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAC300.00514715529.99957732
TGCTTGA7400.025.84422945
CTTATAC8600.024.0847281
TATACAC8750.023.6568093
TTATACA9100.023.4886782
CTTCCGA27250.023.4641171
TCCGATC27150.023.4527073
CGATCTG12300.023.4143035
TTCCGAT27200.023.4095952
CCGATCT27900.022.8222584
CTGCTTG8700.021.98244744
CGATCTT5700.019.7365655
CGATCTC8000.019.6872235
CGATCTA4050.019.444175
ATCTACG1454.76939E-918.6204287
GTATTAG3750.018.6115631
TATTAGA2550.018.529152
TCTGCTT11000.017.79520243
GTATTAA2800.016.8854891
CGCGGTT2800.016.87476210
Produced by FastQC (version 0.11.2)