FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell91.35100000095ae1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell91.35100000095ae1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804358
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC67170.3722653708410415RNA PCR Primer, Index 39 (95% over 21bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA56680.3141283492521994RNA PCR Primer, Index 39 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC54830.303875395015845RNA PCR Primer, Index 39 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG30780.17058698994323743RNA PCR Primer, Index 39 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC13850.024.8699021
TATACAC15400.024.544613
CGATCTG18050.021.8130475
TTCCGAT40200.021.4917962
TCCGATC40250.021.46513
CCGATCT40600.021.2794634
CGATCTT9500.021.0776375
CTTCCGA41300.020.9866491
TTATACA18300.020.0402932
TGCTTGA14100.018.98870845
ACCGTAC851.2054983E-418.5282617
CGATCTA5700.017.7620535
CTGCTTG15200.017.61452344
GGCGTTA2201.2732926E-1116.3639842
TATACTG4500.015.9990045
GACGGCC3850.015.77823911
CGGTACC1602.9668627E-715.46821745
CGATCTC11900.015.3141755
ATACACA25850.015.1441264
TACACAT23750.015.0622225
Produced by FastQC (version 0.11.2)