FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell90.35100000095ac7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell90.35100000095ac7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694433
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC109590.6467650240522936No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC81670.48199014065472046TruSeq Adapter, Index 1 (95% over 22bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA67390.39771416161040296TruSeq Adapter, Index 1 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG51820.30582501639191395RNA PCR Primer, Index 1 (95% over 24bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA30500.18000121574591618No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTT23060.1360927224623222TruSeq Adapter, Index 1 (96% over 27bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG18100.033.06585
TTCCGAT39200.031.166622
TCCGATC39350.030.9906373
CTTCCGA39800.030.9448171
CCGATCT40550.030.2399864
TATACAC22100.029.4226323
CGATCTA5550.028.783355
CTTATAC23300.027.9270841
TTATACA23500.027.765542
CTGCTTG14600.026.50566744
ATCTGCG3350.024.8496387
TGCTTGA16300.024.43142545
CGATCTC10350.023.6952935
CGATCTT10150.022.3888265
GATCTGG13050.021.8962216
CCGCTAG1850.021.89285536
ATATCGT955.5357305E-721.31546837
CGCTAGA1900.021.31546837
TCGAGTA2650.021.22609541
GTACGTA654.2406507E-420.76830513
Produced by FastQC (version 0.11.2)