FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell9.351000000950b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell9.351000000950b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570696
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA40800.15871188191835986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG30250.028.7849375
CGATCTA9800.028.4692045
CTTCCGA65400.028.1967141
TCCGATC65200.028.1938343
TTCCGAT65900.027.9284972
CCGATCT66650.027.5804674
CTTAACG651.5250105E-524.230149
CGATCTT15400.022.3537525
CGATCTC16850.020.8307255
CTAGCGC550.00393843320.4540169
ATCTGCG5700.019.7363327
TCGAGTA2250.018.99950841
TCTTCCG27150.018.729165
ACAATCG2450.018.3668737
GATCTGG20500.018.1096386
GATCTGC13050.018.1033336
GCTCTTC28750.017.5303233
CGTACCG901.8671801E-417.49954610
GATCTAG6750.016.6665576
GATCTCG8850.016.5253166
Produced by FastQC (version 0.11.2)