FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell89.35100000095aae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell89.35100000095aae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617631
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA21410.1323540411873907No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC20360.12586306765881713RNA PCR Primer, Index 27 (95% over 21bp)
ATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACAGTACT20260.12524487970371487No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACAG19250.1190011813571822No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATAG19130.11825935581105951No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC18490.11430295289840513RNA PCR Primer, Index 27 (95% over 24bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA17320.10707015382370887RNA PCR Primer, Index 27 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA406.1667204E-428.12540441
CGATCTG16750.027.6694765
CTTCCGA39500.025.8201351
TCCGATC39850.025.6880743
CCGATCT39950.025.5111434
GTATACG450.001222253125.0158271
GTCGACG551.3684052E-424.54580943
TTCCGAT43050.023.7263642
CGATCTC10500.021.6411615
CGATCTA6150.021.2178525
CGATCTT9950.020.1240545
CGCTATA1403.088644E-919.28658939
CATAACG706.984474E-419.28599243
TATTCGC600.00651107618.75026941
TCTTCCG17450.018.694815
CGGTACC1454.782123E-918.6192345
TGCTTGA5700.018.5511845
TCCGCTA1709.276846E-1118.53025237
CACAACG4050.018.33416424
GAATCGG1253.837522E-717.99914722
Produced by FastQC (version 0.11.2)