FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell88.35100000095a84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell88.35100000095a84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2606822
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA56330.21608686745777042No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG37540.14400676379131372No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC32180.12344532921695459No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC29560.11339477724217456No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT28380.10886819276498357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA7950.029.1498015
TCCGATC50250.028.11833
CTTCCGA50550.027.9728871
CCGATCT51450.027.418754
CGATCTG23550.027.3237695
TTCCGAT51950.027.2414722
GTCGTAT851.8952414E-723.82396737
CGATCTC16000.021.6554015
CGATCTT9950.020.8032045
TCTTCCG21850.019.0495975
CCGTCGA750.001110743117.99998541
GTATAGC1902.3646862E-1117.77611
GATCTCG6850.017.0796226
GCATAGA2550.016.7769181
ACGTCCG2850.016.57829743
GCTCTTC25200.016.517213
TACCGTC3650.016.026777
ATCTGCG4250.015.881737
GATCTGG18700.015.7614156
CGTCGTA3750.015.59938810
Produced by FastQC (version 0.11.2)