FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell86.35100000095a41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell86.35100000095a41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21537
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC428719.905279286808746No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC299413.901657612480847TruSeq Adapter, Index 11 (95% over 22bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA228610.614291684078562TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG17618.176626271068393RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT7143.315224961693829TruSeq Adapter, Index 11 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT5832.7069694014951016No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA5502.553744718391605RNA PCR Primer, Index 11 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC4552.1126433579421464TruSeq Adapter, Index 11 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT4021.8665552305335005RNA PCR Primer, Index 11 (95% over 21bp)
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT1640.7614802433022241RNA PCR Primer, Index 17 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTC1450.6732599712123323No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGTC1090.5061057714630636No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTC880.4085991549426568No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT840.390026466081627No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTC780.3621674327900822No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGTCTTC720.3343083994985374No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG670.31109253842225004TruSeq Adapter, Index 11 (96% over 28bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTAG660.3064493662069926No Hit
TCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCTGCTTGA600.2785903329154478Illumina PCR Primer Index 9 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCTG590.2739471607001904Illumina PCR Primer Index 9 (95% over 22bp)
TCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGTCTTCTGCTTGA570.26466081626967547Illumina PCR Primer Index 9 (96% over 26bp)
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAA480.22287226633235824TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGTCTTCTG440.2042995774713284Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGTC420.1950132330408135No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTTC390.1810837163950411Illumina Single End Adapter 1 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGTCTTC320.14858151088823884No Hit
TCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTTCTGCTTGA320.14858151088823884Illumina Single End Adapter 1 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCT290.13465199424246646No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCGTACTAG220.1021497887356642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC5150.045.01
TTATACA5950.038.949582
TGCTTGA2850.037.10526345
GCTTGAA504.5098204E-836.00000445
CTGCTTG3000.036.044
TATACAC6450.035.5813943
TCTGCTT4050.026.11111343
TTCTGCT4400.023.52272842
ATACACA10300.022.2815534
TACACAT10550.021.7535535
CTTCTGC4950.020.9090941
ACCTCGT550.003814185320.45454627
GACCTCG550.003814185320.45454626
ACTAGAC600.006309710418.75000222
AGACCTC600.006309710418.75000225
CTAGACC600.006309710418.75000223
TAGACCT600.006309710418.75000224
ACACATC12400.018.5080666
CACATCT12950.017.7220087
ACATCTC13400.017.1268658
Produced by FastQC (version 0.11.2)