FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell85.35100000095a27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell85.35100000095a27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607593
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA50530.3143208511109466No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG32350.20123252589430285No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC31410.1953852747554885No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT27060.16832618703863478No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA24080.14978915683260624No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC20690.12870172985326508No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT20230.125840309083207No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA16790.10444185810712041No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACC16660.10363319571558224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA37050.028.1388051
CGATCTG16800.028.1245145
CGATCTA4500.027.9995165
TTCCGAT37650.027.5493262
TCCGATC37850.027.4631963
CCGATCT38100.027.1648854
CGATCTT9950.023.5171835
CGATCTC9500.021.7890995
ATCTGCG2100.021.42827
CGGTACC4200.020.3567945
CCCAACG1552.3646862E-1120.3222321
CTACGGG1552.3646862E-1120.3222338
TCTTCCG15100.019.9665435
GCTCTTC15850.019.305663
CGGTCCG1051.4465404E-619.28538139
AGCGTCG600.00651225118.74967437
TCGAGTA1202.4986548E-718.74967441
GATCTGT4950.018.6360426
TGTGCGA750.001110613917.9996910
CGGGCTA1751.382432E-1017.9996941
Produced by FastQC (version 0.11.2)