FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell84.35100000095a0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell84.35100000095a0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113821
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA36810.1741396267706679No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC23090.10923346868064987No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG22120.10464462222676375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA450.001227162524.99906740
CGATCTG24700.024.5942235
TCCGATC57800.024.406533
CTTCCGA58550.024.1563191
CCGATCT58350.024.0993584
TTCCGAT58800.024.0297152
CGATCTA8650.022.1090035
CGATCTT14450.021.1756825
TGCGATT550.00393684720.45523530
CGATCTC15950.019.1842375
ACGTCCG951.3171093E-518.94666143
CGGACGT750.001110568418.00017736
CGAGTAT750.001110920917.99932720
ATCTGCG2850.017.3677737
CCCAACG1205.1790776E-616.8743721
CGGTACC4400.016.8743745
TTAACGG4500.016.50016435
GTCTATA2403.6379788E-1215.9527551
GCCGTAT850.00256635415.88213639
CGGGTAT1004.170286E-415.74941315
Produced by FastQC (version 0.11.2)