FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell83.351000000959e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell83.351000000959e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285605
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT18550.022.0743245
TCCGATC69450.021.3487823
CGATCTA10350.021.303245
TTCCGAT70150.021.1036782
CTTCCGA70400.021.0782171
CCGATCT70100.021.0545394
CGATCTG28400.020.1221945
TCGACGG908.260824E-619.9989644
ACGATAG706.9888076E-419.2847145
GTCGACG951.3173527E-518.94638343
CGTCGAC1002.0486053E-517.99945842
ATAGTCG750.001111067517.9990656
TATCCCG750.001111067517.9990655
CCGTCGA1053.1148196E-517.14271541
TATTAGG4300.016.2200872
GTATTAA6700.015.7958921
CGACGGC1301.1078921E-515.57611345
TAACGCC2505.456968E-1215.2992034
ATAACGC2800.015.2670623
GCATTCG6100.014.7533322
Produced by FastQC (version 0.11.2)