FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell82.351000000959d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell82.351000000959d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381390
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA26910.11300123037385729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT65200.022.3275682
TCCGATC65350.022.241893
CGATCTG28700.022.1860585
CTTCCGA66600.021.9382251
CCGATCT66550.021.8403744
CGATCTA8350.021.8260025
CGATCTT15950.021.3006045
GGTTCGC706.988213E-419.2850028
CGTCGTA2150.017.79004110
TTAACGG2700.016.667135
CGGGTAT952.8217415E-416.57833515
ACCGTCG2201.2732926E-1116.3630338
ATCTGCG2800.016.0708357
TACCGTC2251.8189894E-1115.9994117
CCGTCGA850.002566519215.88210241
TCGTCTA850.002566519215.88210223
CGTCGAC850.002566519215.88210242
CGGTACC2850.015.78889245
GTATTAG8050.015.381471
CGCCGTA1209.758714E-515.00007625
Produced by FastQC (version 0.11.2)