FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell81.351000000959bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell81.351000000959bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235472
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG29750.022.4615825
TCCGATC68150.021.6905383
CTTCCGA68300.021.6593761
TTCCGAT68250.021.6257932
CCGATCT68950.021.4715044
CGATCTA8700.019.1374175
CGATCTT17450.019.0825825
GGCGTTA2400.017.8124242
CGATCTC19750.016.062865
CGTTCGG850.002566641415.88192745
GATCTGG15200.015.838396
GTATTAA5950.015.13715651
GTATTAG7450.015.1117571
CGAAACG1056.041255E-414.99959719
GCGTTAT2900.014.74131343
CGCGAAG2651.6370905E-1114.43357622
TACGGGT1453.1077245E-513.9651424
GATCTGT11200.013.8612356
CGGGTAT1301.928215E-413.84547215
CTTACAC3950.013.6705193
Produced by FastQC (version 0.11.2)