FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell80.35100000095992.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell80.35100000095992.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1642378
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA22420.13650937847438288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG20100.025.2978575
CGATCTA6300.024.6422215
TCCGATC45050.024.3223483
CTTCCGA45550.024.1696071
TTCCGAT45550.024.1541542
CCGATCT45350.024.0126114
ATACCGC2650.018.67990327
GTACTAA1951.8189894E-1218.4728761
CGATCTT11450.018.471145
ACAATCG1002.0468842E-518.00063337
TCGAGTA1002.0474205E-518.00008641
CGATCTC12350.017.489435
CAATCGA1053.1136093E-517.14293938
GATCTGG10350.016.73876
GCTATAC1358.590214E-716.6662393
TTAACGG1803.910827E-916.25057235
ATCTGCG1803.916284E-916.2490887
CGTCGTA2251.8189894E-1115.99910210
GATCTGT7050.015.9570366
CGGTACC2009.295036E-1015.74911645
Produced by FastQC (version 0.11.2)