FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell8.3510000009509b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell8.3510000009509b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638202
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA19110.11665228097633869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA39450.026.4856871
CGATCTG17600.026.2073675
TCCGATC40200.025.8581923
CCGATCT40300.025.849864
TTCCGAT40250.025.8260692
CGATCTA4950.025.4545295
GCTTACG500.00226502722.4999852
CGATCTT9900.022.2727135
CGATCTC11550.020.6493385
TAGACCG550.00393725820.454535
CGCCTAT1452.1827873E-1020.172436
TCTTCCG17800.019.5926845
ATCTGCG2400.018.7494147
GCTCTTC19700.018.1598853
CGATTAC901.8667335E-417.49945445
GGGTTAG4550.016.8126627
TAGGGGT4500.016.4999894
TCTATCG1501.423814E-716.49998931
TCGCCTA1652.3601388E-816.36362535
GGGGTTA4400.016.3636256
Produced by FastQC (version 0.11.2)