FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell79.35100000095978.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell79.35100000095978.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305215
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA30200.1310073030064441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG23550.025.9855845
TCCGATC58500.024.8060913
TTCCGAT58800.024.7560542
CCGATCT59200.024.3987624
CTTCCGA59900.024.203981
CGATCTA10700.023.7601475
CGGGTAT1152.6557245E-1023.47674615
CGTCGAC602.468908E-422.49952342
TCGACGG706.988402E-419.28488744
CCGTCGA851.2047025E-418.53022641
CGATCTT14100.018.1903155
GTCGACG750.001110842217.99961943
ATCTGCG2150.017.7895497
CGATCTC18600.017.660155
TTAACGG3100.017.42011835
CCGGGTA1853.074092E-1017.02592714
CTAACGC800.001712289416.873918
GTATATA8200.016.747261
GTATTAG9400.016.285791
ATAACGC3000.015.7489823
Produced by FastQC (version 0.11.2)