FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell78.3510000009595f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell78.3510000009595f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151405
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA24320.11304240717112769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT709.3176095E-725.71310415
CTTCCGA50500.024.5689321
TCCGATC50150.024.5402493
TTCCGAT50300.024.4223372
CCGATCT50250.024.4018634
CGATCTG21300.024.0833995
CGATCTA7150.022.6563015
CGATCTT14050.021.2979535
CGTTCCG600.006514028618.7491389
CGATCTC14100.018.3502225
CCGATCG4350.017.58539814
CGATCGC4400.017.38556515
ATCTGCG2400.016.8742247
TCGCACG4350.016.55096218
GTATTAG7650.016.1892721
GATCGCA4750.016.10452316
GTCTATA3000.015.7624651
TAGCGTA2505.456968E-1215.2992967
CGCGCAA2651.8189894E-1215.28231721
ACGCCCC5050.014.70229422
Produced by FastQC (version 0.11.2)