FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell77.35100000095928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell77.35100000095928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750253
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA5500.026.1817195
TTCCGAT41650.025.4981042
TCCGATC41700.025.3596173
CTTCCGA42050.025.3235281
CCGATCT42350.024.9703884
CGATCTG17900.024.0082875
CGATCTT10300.023.810595
CGATCTC12800.019.1600845
AACGTCC750.001110780317.99941641
GTATTAG5800.017.078661
CGACCGA800.001711698516.87445420
GGCGTTA2005.0931703E-1116.87445342
TTAACGG1803.912646E-916.25040235
TAATACG1156.785964E-515.6521154
GATCTTT10650.015.6337426
TATAGAC2600.015.5768633
TACGACG2051.3187673E-915.3657955
ATAATAC6750.015.3332763
GATCTGG12750.014.6470046
GTATAAA5100.014.1256651
Produced by FastQC (version 0.11.2)