FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell76.3510000009590e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell76.3510000009590e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213755
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA24630.11125892431637648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA52350.026.540831
CGATCTG26750.026.2430175
TTCCGAT53600.025.9002112
TCCGATC54400.025.4779663
CCGATCT54700.025.29714
CGATCTA7950.025.1887055
CGATCTT11300.020.90715
TCTTCCG24250.019.4845545
GCTCTTC26050.018.0518423
GTCGACG750.001110825917.9996143
CGATCTC14250.017.6842295
CGAGCGT901.8666472E-417.5000176
GATCTGT9150.017.213136
GTATTAG7700.016.6697921
CGCCGTA952.8212988E-416.5785925
GTGCTCT28750.016.5268731
TCTATCG1004.1698993E-415.7496629
GATCTGG18450.015.7317226
TGCTCTT31900.015.0235252
CGTCGAC900.003755922414.99967742
Produced by FastQC (version 0.11.2)