FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell75.351000000958f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell75.351000000958f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369050
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA66360.19696947210638013No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAAT52600.15612709814339354No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT40270.11952924414894406No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG38770.11507695047565338No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC37230.11050592897107493No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG36640.1087546934595806No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT34280.10174975141360323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG44050.029.521825
CTTCCGA90050.029.0031341
CCGATCT93150.028.0179464
TCCGATC94700.027.7019143
CGATCTA12700.026.0420285
TTCCGAT102300.025.6658952
CGATCTT19650.023.930125
CGATCTC24200.021.5691875
GATCTGG31350.020.1664736
ATCTGGG19750.019.02417
TCTTCCG36100.018.9464385
GATCTGT9900.018.6354486
ATTCGCG1102.2567892E-618.41064530
CGGGTAT1102.2599925E-618.40791315
CGGTTAA1608.1854523E-1018.2800842
GATCTAG8300.017.8906886
GCTCTTC38000.017.8214933
TCTATGC6600.017.72643
GATCTGC17700.017.541516
GATCTCG12700.017.538516
Produced by FastQC (version 0.11.2)