FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell74.351000000958d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell74.351000000958d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491640
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA25970.17410367112708158No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC15520.10404655278753586No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG15180.10176718243007697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG17150.026.7615155
CTTCCGA39450.026.3107681
TCCGATC39950.025.9051273
CCGATCT40100.025.8082244
CGATCTA5850.025.7669855
TTCCGAT41700.024.8179822
CGATCTT8800.020.7084225
CGATCTC12100.020.2668275
TCTTCCG17350.019.7100965
TCTATCG951.3153476E-518.94889331
ACGTCCG600.00651088118.7502543
CTATCGC1002.045882E-518.00144832
TATCGCC1002.045882E-518.00144833
CTCGTAT750.001110084418.00084539
TCGTATG750.001110084418.00084540
CGTCGGC1751.382432E-1018.00084341
GACCGTA901.8654771E-417.50082226
GTATTAG6250.016.931581
CGAACTA1601.6128979E-816.87635829
GGACCGT800.001712165816.873536
Produced by FastQC (version 0.11.2)