FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell73.351000000958bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell73.351000000958bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598670
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA24780.15500384694777533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG15050.029.1509195
CTTCCGA34100.029.049441
TCCGATC35400.027.8380363
CCGATCT35500.027.7587494
TTCCGAT36250.027.185282
CGATCTA4850.025.5137985
GTCGACG450.001226560725.00071343
CGATCTC10700.022.9190625
CCGTCGA602.4676195E-422.5006441
TCTTCCG13250.020.8854275
GCTCTAT3300.020.466651
CGATCTT9500.019.4197855
ATAGCGT706.9881737E-419.2844566
ATATCGC706.9881737E-419.2844566
GCTCTTC14800.019.0027313
AACGCAT1709.276846E-1118.5299435
TCTATGC3850.018.1162663
GTCTAGG1507.21775E-918.0106531
GTTCGCC750.001110968417.99882316
GGCGTTA1406.478513E-817.67907542
Produced by FastQC (version 0.11.2)