FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell72.35100000095886.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell72.35100000095886.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023334
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA53530.17705619028529432No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG32040.10597572084328095No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC30890.10217197306020441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG29650.030.4294135
CTTCCGA62250.030.1297631
TTCCGAT63400.029.5972042
TCCGATC63600.029.5041313
CGATCTA9750.029.307115
CCGATCT64750.028.771634
CGATCTC17250.021.521315
CGATCTT14850.020.454145
TCTTCCG24900.018.6140885
ATCTGCG4950.017.7266277
GCTCTTC27950.016.9048523
TTAACGG5350.016.82237435
GATCTCG8450.016.5085496
GATCTGG22150.016.3540766
GATCTAG7050.016.2762746
GATCTGC16950.016.194376
TCCCGTA850.002567086115.88177512
CGGTACC6000.015.74942845
TATTAGA9500.015.394432
CGCCTAT2001.5761543E-814.62519334
Produced by FastQC (version 0.11.2)