FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell71.3510000009586c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell71.3510000009586c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790351
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA21950.12260165744035667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA8100.023.8879995
TCCGATC54600.022.7876133
CGATCTG21200.022.7114185
CTTCCGA55600.022.5155121
CGGGTAT802.9772546E-622.49916315
TTCCGAT55550.022.4384082
CCGATCT55150.022.3971714
ACGTCCG654.2405687E-420.7684643
CGATCTT14750.020.5924555
CGCCTAT1403.090463E-919.28607236
ATATCGG706.987304E-419.2849948
TACCGTC2200.018.4084057
CGCCGTA750.001110621417.99983239
GTGCGTA750.001110621417.99983238
TCGCCTA1651.2314558E-917.72760235
ACCGTCG2105.456968E-1217.1422188
TACGGGT800.001711775116.8743724
CGATCTC15650.016.6766955
ATCTACG1803.916284E-916.2493957
CGTCGTA2251.8189894E-1115.99940510
Produced by FastQC (version 0.11.2)