FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell70.35100000095843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell70.35100000095843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115746
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.7732448637533911No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7150.617731930261089No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.6004527154286109No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.5745338931798939No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGACG1780.1537850120090543Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT1610.139097679401448Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCACTGGGAGACGTGTGCTCTTCCGATCTGCACTGGGAGACGTGTGCTCTT1290.11145093566948318Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCAGGGAGACGTGTGCTCTTCCGATCTGCCCAGGGAGACGTGTGCTCTT1170.10108340676999636Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCCC250.002101282435.99308445
TTCTAGA300.00513318829.994241
TCTGACC706.9406227E-419.2820118
GCGGGGA600.00648317218.74639913
ATCTCCT1053.06716E-517.14697526
TCTTTTT14000.015.5862918
ATCTTTT13900.015.5365847
GATCTTT13900.015.3747446
CTAGACG1351.560116E-514.9971183
CTTTTTT14700.014.8440889
CTCCACA950.005328942614.2139438
TAGACGT2306.4519554E-913.6930224
CCACAGG1000.00748530613.503242540
TCCACAG1000.00748530613.503242539
CGATCTT18400.013.3262445
TCTGAGC1403.5687807E-412.8546738
ACACATC1250.002233635212.5975845
CTCAGGG1454.7806973E-412.41677431
ATCTGAC2207.7334334E-712.27037057
TCTAGAC1701.3597435E-411.9094762
Produced by FastQC (version 0.11.2)