FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell7.35100000095064.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell7.35100000095064.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662564
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA39560.23794572720208063No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC23210.1396036483407556No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG22960.13809994682911453No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT21060.1266718153406425No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA18170.10928902586607192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG19150.028.0806035
TCCGATC44700.027.1305453
CTTCCGA45000.027.0675851
CCGATCT45150.026.8601424
TTCCGAT45600.026.5950762
CGATCTA6650.024.698955
CGATCTC13200.022.3292775
CGATCTT10350.021.7388695
CGGTACC3000.020.99911545
GATCTAG3950.019.9364686
GATCTGT6150.019.390016
TCTTCCG20050.019.3015165
CGTAAAT951.3168867E-518.94657115
GGTATCG600.006512141318.74977539
GATCTGG14050.018.736436
ATCGATA750.001110372618.00032423
CGACCGT750.001110820917.9992419
GCTCTTC21800.017.9584983
ATCTGCG2200.017.3856327
ACGTCCG1305.78897E-717.30696343
Produced by FastQC (version 0.11.2)