FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell69.35100000095836.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell69.35100000095836.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899109
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG450.001227209324.99874310
TATCCCG1052.3701432E-923.5708645
TCCGATC53400.023.3842723
TTCCGAT53400.023.3842722
CGATCTG22650.023.1451385
CTTCCGA54200.023.059151
CGATCTA8400.023.0351625
CCGATCT54550.022.7675574
CGATCTT12800.020.3901355
TCTATCG750.00111017618.0009929
CGATCTC15750.017.1424475
ATCTACG1752.6957423E-916.7134467
AATCGTC850.002565050315.88322628
CGCTAGA4350.015.51686937
GCATTCG5000.014.84925222
ATTCGTA4850.014.84500524
ACGACGG2601.0913936E-1114.7111856
CGACGGT2601.0913936E-1114.7107997
GACGGTA2601.0913936E-1114.7107998
TTCGTAT5050.014.70261825