FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell68.3510000009581c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell68.3510000009581c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675033
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA4900.024.3372045
TTCCGAT45300.023.1461412
TCCGATC45600.022.9445233
CTTCCGA46100.022.8116681
CCGATCT45900.022.7945564
CGATCTT9900.022.5004335
CGATCTG22100.022.5004315
ATCTGCG2450.018.3660567
TGTGCGA750.001111035917.99873410
GTATTAG5100.016.3370111
CGATCTC13800.015.9785685
GATCTGG12150.015.9262326
ATAACGC2453.6379788E-1215.6125463
ATTCGTA3200.015.46904824
GTATTAC2701.8189894E-1215.0123891
TAACGCC2253.0559022E-1015.0002894
GTCCTAT4700.014.8526821
GTATAGA2751.8189894E-1214.7394361
CGCATCG2304.2564352E-1014.67288213
ACCGTCG1705.8969636E-714.55788
Produced by FastQC (version 0.11.2)