FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell67.351000000957f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell67.351000000957f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803561
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGT300.005147824530.000244
CGATCTA5300.026.3209655
TCCGATC39550.025.1455883
CTTCCGA39700.025.011931
TTCCGAT39700.024.9939022
CCGATCT40750.024.4051024
CGATCTG17550.023.7181385
CGATCTT11550.020.454715
GATCTAC3850.019.2858686
CGATCTC10950.018.904265
CTAGTAC1002.047544E-518.0001453
CGCGGAA800.00171158116.87466845
GTATTAG6500.016.6275021
ATCTGCG2750.015.5451497
ACACCGC1056.0388533E-415.000126
TATCCGT900.003755516414.99970410
TTAACGG1502.576E-614.99970335
TATACTG5000.014.8501195
ATACTGT6450.014.651286
GTATATA6850.014.4630961
Produced by FastQC (version 0.11.2)