FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell66.351000000957d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell66.351000000957d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639165
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA6300.026.0713065
TCCGATC38500.024.8959853
CTTCCGA38550.024.7613161
TTCCGAT38800.024.5875132
CCGATCT39100.024.3413164
CGATCTG16050.024.2522225
CGATCTT9100.021.5108875
ATCTGCG2500.018.8993347
GGACCGT750.00111053317.9999146
CGATCTC11500.017.9999145
CGCCTAT901.8663575E-417.49991634
GTATTAA4550.017.3176481
GATCTAG3800.017.170976
TATCGCC1004.1675748E-415.75040531
TACGCAA1004.1695096E-415.74944410
GTATTAG6050.015.2567041
CTATACC8950.015.0837284
ACCGTCG1951.1283191E-814.9994728
CGGGTAT900.003755711714.99947115
CGGGCTA1056.040421E-414.99947141
Produced by FastQC (version 0.11.2)