FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell65.351000000957a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell65.351000000957a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592387
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC32700.025.871073
CCGATCT33300.025.540064
TTCCGAT33100.025.4904562
CGATCTG14300.025.489035
CTTCCGA33300.025.3596571
CGATCTA4500.024.4995385
ACCGTCG1700.023.8223348
CGATCTT9300.020.806065
CCGTCGA654.23886E-420.76949141
CGTCGTA1900.020.13056810
CGATCTC9450.019.9996245
TACCGTC2000.019.1240397
TCTTCCG16050.017.8034025
ACCGTAC1153.452009E-617.60781117
CCGTCGT2200.017.385499
GGATCGT800.001711811816.87415343
GTATTAA6150.016.8437611
ATACCGT2301.8189894E-1216.6301216
ACACCGT1904.4929038E-1016.5786366
TAAACGC2101.07320375E-1016.0716328
Produced by FastQC (version 0.11.2)