FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell64.35100000095787.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell64.35100000095787.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000245
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA28550.1427325152668798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA300.005149180529.9987812
CTTCCGA49700.028.0409261
CGATCTG24000.028.0301135
TCCGATC49850.027.8474163
TTCCGAT49850.027.8474162
CCGATCT50750.027.3092374
CGATCTA6750.026.3322645
CGATCTT12450.022.2280165
CGATCTC12050.022.0323035
ATCTGCG3600.019.3742147
GTCGACG851.2053814E-418.5286643
GATCTGG16150.018.1107226
TCTTCCG20850.017.9129435
GTATTAA5250.016.7248971
GATCTGC12850.016.6335666
CCGTCGA952.8204606E-416.57910341
CGTCGAC1004.1702506E-415.74936142
GCTCTTC23700.015.6639213
CGCCGTA1156.7859684E-515.65232325
GATCTAG5750.015.651546
Produced by FastQC (version 0.11.2)