FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell63.3510000009576d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell63.3510000009576d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770314
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA21120.11930086978920124No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACAC18590.10500961976236983No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTT17820.10066010888463855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA46050.027.8201081
TCCGATC47200.027.123893
CCGATCT47300.027.0189764
CGATCTG21800.026.628395
CGATCTA6900.026.4129945
TTCCGAT48800.026.37292
CGATCTT12550.022.051755
CGATCTC11400.021.7104845
CGCTATA1700.021.1764339
TCGACAC2000.020.2499623
ATTCGAC2150.018.849951
TCTTCCG18550.018.5579175
AAGTTCG3650.018.49311636
GATCTGC8850.018.305056
CGGTCAA3650.017.87617318
GATCTGA7750.017.7096426
TACCGTC1953.45608E-1117.3071737
ACCGTCG1853.074092E-1017.0265148
AGCCGGT3850.016.9475415
TCCGGGT2850.016.57844714
Produced by FastQC (version 0.11.2)