FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell62.35100000095744.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell62.35100000095744.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804184
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA44290.24548493945185193No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG28210.15635877493648098No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT23690.1313058978463394No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC22180.12293646324321689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG21700.028.5131235
TTCCGAT46950.027.2684562
TCCGATC46900.027.2495523
CCGATCT47500.026.7151324
CTTCCGA47950.026.6742821
GTCGACG608.196499E-626.24935343
CGATCTT10250.023.267725
CGATCTA6250.023.0394335
CGGTAAG602.468214E-422.50006928
CCGTCGA702.7046106E-522.49944541
CGATCTC12500.021.5994685
CGTCGAC754.6053727E-520.99948342
GATCTGG14850.019.9995086
TCGACGG807.567482E-519.68701444
GCATTCG3650.019.10911822
CAATCGA600.00651282818.74953838
TCGGTAA750.001110533518.00005527
CGTTCGG750.0011107417.99955724
ACGTCCG1253.8372127E-717.99955743
ATCTGCG3000.017.2495757
Produced by FastQC (version 0.11.2)