FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell61.3510000009572a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell61.3510000009572a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995630
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA62050.3109293806968226No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG38210.1914683583630232No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC37160.1862068619934557No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT31620.15844620495783288No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA29010.14536762826776506No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT22600.11324744566878629No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT22530.11289667924414848No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC22390.1121951463948728No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA21970.1100905478470458No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC20070.1005697448925903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAG406.1661826E-428.1262228
CGATCTG18900.025.3557015
CTTCCGA47300.024.0865041
TCCGATC48150.023.5500663
CCGATCT48150.023.5500664
TTCCGAT49300.023.0463562
CGCTATA1305.0931703E-1122.50097739
CGGTACC4500.021.4987845
CGATCTT12400.021.0471925
TCGGTAA550.003936547320.45543527
CGACGCA550.003938261420.45389743
CGTATCA550.003938833320.45338445
CGATCTA7700.020.4533845
CGCGGTA4950.019.99936543
CGATCTC12550.019.0029055
CGTTCGG600.00651238918.74987624
GCGTATC600.00651427318.74893444
TAACGGC5300.018.68005636
CCTTGCG6300.018.57223333
CGGACCT851.2055296E-418.5283619
Produced by FastQC (version 0.11.2)