FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell60.3510000009571d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell60.3510000009571d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188589
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA31550.144156806051753No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAA30200.13798844826506942No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCTA26740.12217917571549523No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCAA26700.12199640955885276No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAAC23620.1079234154973821No Hit
CACATATACTATCTGGAAAAGGCCAATAAAATTAAATCTGAGAAAACACAC22750.10394825159040824No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCTC22290.10184644078901978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG25400.029.053455
CTTCCGA53900.028.572781
TCCGATC55650.027.612973
TTCCGAT56250.027.3984282
CCGATCT55950.027.3844854
CGATCTA8650.026.5302725
CGATCTT11500.023.4769135
ATCTGCG4050.021.1094197
GGCGTTA2050.019.75586742
TACCTAG951.3176312E-518.9458487
CCCGTCG2500.018.90107539
GTCTATA2150.018.8503421
GATCTGT7100.018.696116
GATCTGG15950.018.6196216
CGCGAAG2450.018.36713222
GATCTGC12300.017.7428846
GTATTAG6150.017.5732171
TCTTCCG22700.017.4439335
GATCTCG5950.017.0158316
GCTCTTC23150.017.0076643
Produced by FastQC (version 0.11.2)