FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell6.3510000009504b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell6.3510000009504b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938895
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA21760.11222887263106048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA40250.029.648161
TCCGATC40900.029.0451413
CGATCTA5250.028.7129845
CGATCTG19100.028.3887675
TTCCGAT42000.028.2308642
CCGATCT41900.028.137154
CGATCTC11100.024.5259155
CGATCTT10850.020.7363875
TCTTCCG16050.019.9056425
ATCTGCG3100.019.595387
ACGCGCT706.9831195E-419.28682929
GTATTAG7700.019.0068631
CCGTCGA600.006511835418.75011841
GTCGACG600.00651377518.74915143
TCTCGAC1651.2332748E-917.7260138
GCTACGA1305.790389E-717.306912
ACCGCCT2750.017.18059712
TCGATTA1053.1161057E-517.14163844
CGAGCGT1205.1790266E-616.8742356
GATCTGC11600.016.8742356
Produced by FastQC (version 0.11.2)