FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell59.351000000956f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell59.351000000956f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914996
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA40300.1382506185257201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG35500.022.5626225
CGATCTA11950.022.2168275
TCCGATC83750.022.0559753
CCGATCT84450.021.9264414
TTCCGAT84250.021.8983752
CTTCCGA84600.021.8516181
CGATCTT19250.020.1032225
CTACGCT750.001111157717.9990869
TACCGTC3950.017.0877427
CGATCTC24850.016.4783215
ATACCGT4150.015.7223646
CGGGTAT2302.5465852E-1115.65138115
GATCTGT13750.015.5449346
ATCTGCG4400.015.3401317
GGTACTA4850.015.30929139
ACCGTCG4300.015.173658
CGTCGTA4050.014.99924110
ATCTCGT4250.014.8227787
CCGGGTA2304.2564352E-1014.67316914
GATCTGG19350.014.1855716
Produced by FastQC (version 0.11.2)