FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell58.351000000956d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell58.351000000956d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250921
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA30810.13687730488986508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG28900.021.4880375
TCCGATC74700.020.1808623
TTCCGAT74950.020.113552
CTTCCGA75250.020.1043261
CGATCTA11100.020.0677075
CCGATCT74700.020.0603794
CGATCTT16700.019.1318685
ACCGTCG2500.017.099368
TACCGTC2550.016.7640767
ACGTCCG1257.632176E-616.20011143
GCATTCG5100.015.88211122
CCGCTAG5150.015.72826536
CGTCGTA2600.015.57633910
CGCTAGA5350.015.56085537
GCTATAC2559.094947E-1215.0001043
CGTATTG5800.014.74148127
CGATCTC23350.014.6467825
GATCTGG15100.014.602756
ATTCGTA5750.014.47803924
ATCTACG2509.640644E-1114.399467
Produced by FastQC (version 0.11.2)